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MiR-200b and Sp1 are co-expressed and inversely correlated with Zeb1 during mouse embryonic development. Paraffin sections of E13.5 mouse embryos were assayed for miR-200 expression by in situ hybridization using LNA probes, or Sp1 or Zeb1 expression using immunohistochemistry. Scale bars indicate 100 μm. Black arrows indicate positive staining, and red arrows indicate negative staining regions.
