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. 2014 Mar 4;289(16):11206–11218. doi: 10.1074/jbc.M113.538546

FIGURE 4.

FIGURE 4.

Intracellular loops of M3 muscarinic receptor bind PLCβ3. A, fragments from the intracellular surface of M3R were expressed as GST fusion proteins demarcated as follows: M3Ri1, 92–104; M3Ri2, 165–184; M3Ri3N, 252–389; M3Ri3M, 352–469; M3Ri3C, 389–491; M3R/H8, 547–560; M3R/CT, 564–590; and M3R/H8-CT, 547–590. Each fragment was tested for binding to purified PLCβ3 as described under “Experimental Procedures.” Representative Western blots shown were from three independent experiments. B, M3Ri3 loop subfragments. C, the indicated fragments from B were tested for binding to purified PLCβ3. Representative Western blots shown were from three independent experiments. D, M3Ri3Na was tested for binding to various purified PLCβ3 proteins (FL; ΔPDZ, 1–1230; ΔCT, 1–886; each at 3 nm). Representative Western blots shown were from three independent experiments. IB, immunoblot.