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. 2014 Feb 28;289(16):11219–11229. doi: 10.1074/jbc.M113.535989

FIGURE 1.

FIGURE 1.

Overexpressed SRC-3 promoted bladder cancer cell proliferation. A, Western blot analysis of SRC-3 protein expression in four bladder cancer cell lines and 11 pairs of human UBC samples. N, non-tumorous bladder tissue adjacent to UBC; T, tumor tissue. B, overexpression of SRC-3 in human UBC samples at the mRNA level. Compare the mRNA level of src-3 between normal (n = 14) and UBC samples (Tumor, n = 46) in the GEO profile dataset (GSE3167). Quantitative RT-PCR assay detected at the src-3 level in 9 human UBC samples. C, cell growth curve in SRC-3 overexpressed and control cells by the MTT assay over 4 days. D, Western blot analysis of SRC-3, Cyclin D1, and PCNA protein levels in SRC-3 overexpressed and control cells. E, colony formation capacity in soft agar of SRC-3 overexpressed and control cells. CTRL, BIU87 cells transfected empty vector; #1 and #2, two different clones from BIU87 cells stably transfected with the SRC-3 expression plasmid. F–K, SRC-3 deficiency in human UBC T24 (F-H) and 5637 (I–K) cells impairs cell proliferation and colony formation capacity. F and I, cell growth curve in SRC-3 knockdown (shSRC-3) and control (shCTRL) cells by MTT assay. G and J, Western blot analysis of SRC-3, Cyclin D1, and PCNA protein levels in SRC-3 knockdown and control cells. H and K, colony formation capacity in soft agar of the SRC-3 knockdown and control cells. **, p < 0.01; ***, p < 0.001.