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. 2014 Mar 10;289(16):11253–11261. doi: 10.1074/jbc.M114.554311

FIGURE 1.

FIGURE 1.

Histone acetylation selectively up-regulates GnT-IX. A, Neuro2A cells were treated with DMSO (vehicle), 0.1 μm TSA for 24 h, and the mRNA levels of GnT-III, -V, and -IX and Fut8 were quantified and normalized to those of rRNA. The mRNA/rRNA levels of TSA-treated samples relative to those of DMSO-treated samples are shown (n = 3). B, Neuro2A cells were treated with DMSO (vehicle), 0.1 μm TSA for 24 h, and the levels of H3Ac around the transcriptional start sites of GnT-III, -V, and -IX and Fut8 genes were analyzed by ChIP assay (n = 4). The precipitated DNA was analyzed by real-time PCR, and the amounts of DNA relative to those with control IgG are shown as -fold enrichment. All graphs show means ± S.E., *, p < 0.05, **, p < 0.01, Student's t test.