TABLE 1.
Plasmids used in this study
The mutation introduced into Env7 CPCS is shown in boldface type and underlined.
| Plasmid | Mutation introduced | Genotype | Source/Reference |
|---|---|---|---|
| pSMG17 | WT | 2 μm URA3 PPGK-ENV7::3xHA | Ref. 35 |
| pSMG18 | WT | CEN URA3 PGAL1-GFP::ENV7 | Ref. 35 |
| pSMG19 | WT | CEN URA3 PGAL1-ENV7::GFP | Ref. 35 |
| pSMG20 | SCC | 2 μm URA3 PPGK-C13SENV7::3xHA | This study |
| pSMG21 | CSC | 2 μm URA3 PPGK-C14SENV7::3xHA | This study |
| pSMG26 | SSC | CEN URA3 PGAL1-GFP::C13/14SENV7 | This study |
| pSMG30 | SSS | 2 μm URA3 PPGK-C13–15SENV7::3xHA | Ref. 35 |
| pSMG31 | SSS | CEN URA3 PGAL1-GFP::C13–15SENV7 | Ref. 35 |
| pSMG33 | CEN URA3 PGAL1-GFP | Ref. 35 | |
| pSMG35 | CCS | 2 μm URA3 PPGK-C15SENV7::3xHA | This study |
| pSMG36 | SSC | 2 μm URA3 PPGK-C13/14SENV7::3xHA | This study |
| pSMG37 | CSS | 2 μm URA3 PPGK-C14/15SENV7::3xHA | This study |
| pSMG38 | SCS | 2 μm URA3 PPGK-C13/15SENV7::3xHA | This study |
| pSMG39 | CSS | CEN URA3 PGAL1-GFP::C14/15SENV7 | This study |
| pSMG40 | SCS | CEN URA3 PGAL1-GFP::C13/15SENV7 | This study |
| pSMG41 | CSS | CEN URA3 PGAL1-C14/15SENV7::GFP | This study |
| pSMG42 | CVS | 2 μm URA3 PPGK-C14V/C15SENV7::3xHA | This study |
| pSMG43 | CIS | 2 μm URA3 PPGK-C14/IC15SENV7::3xHA | This study |