FIGURE 4.

Blockade of Fas signaling in breast cancer cells enhances the number of activated T cells in vivo. A–C, 5×10⁵ 4T1/Fas-WT, 4T1/Fas-DN, and parental 4T1 cells were inoculated s.c. into the flank of BALB/c mice. 14 days later peripheral blood, spleen, and tumor tissues from normal or tumor-bearing mice were collected, and then 1 × 10⁶ cells were stained with FITC-CD3, PE-Cy5-CD4, and APC-CXCR3 for CD3⁺CD4⁺CXCR3⁺ T cells and FITC-CD3 and Percp-CD8 for CD3⁺CD8⁺ T cells, respectively. The percentage and number of CD3⁺CD4⁺CXCR3⁺ T cells and CD3⁺CD8⁺ T cells in peripheral blood (A), spleen (B), and tumor tissues (C) were analyzed by FACS, and the results are represented as the mean value ± S.E. of three independent experiments with similar results. *, p < 0.05; **, p < 0.01.