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. 2014 Mar 13;289(16):11522–11535. doi: 10.1074/jbc.M113.525014

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Higher expression of Fas in human breast cancer tissue correlates with poorer prognosis in breast cancer patients. A and B, protein expression of Fas in human breast cancer tissue derived from Cohort 1 and Cohort 2 was detected by IHC. One representative image for lower or higher expression of Fas is shown. The bar represents 100 μm (A, left panel). The mean intensities of Fas in tumors was measured by defining regions of interest using automated cell acquisition and quantification software for IHC (Histoquest) in a FACS-like manner of scattergram analysis (A, right panel). Higher levels of Fas in human breast cancer tissues were correlated with reduced overall survival in breast cancer patients. Kaplan-Meier survival curves of overall survival in Cohort 1 (B) survival data were analyzed by log-rank statistics, and the p value is indicated.