Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 28;4:22. doi: 10.1186/2045-3701-4-22

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Han et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Conditioned medium from the culture of inflammatory cytokine-stimulated MSCs induced autophagy in HCC cells. (A) SMMC-7721 cells were transfected with GFP-tagged LC3; after 24 hours transfection, cells were incubated with contidioned medium collected from MSCs which were pretreated with inflammatory cytokines or not. Images were taken under a fluorescence microscope. Arrows show the punctate GFP-LC3 in the cytoplasm. (B) The number of punctate GFP-LC3 in each cell of SMMC-7721 was counted and at least 100 cells were included for each group. The results were shown as means (±SD) (*p < 0.05). (C) Electron micrographs showing the ultrastructure of SMMC-7721 cells with or without conditioned medium collected from MSCs pretreated with inflammatory cytokines or not. Arrows indicate the autophagic vacuoles in the cytoplasm. Magnification, ×10,000.