Table 1.
Differentially expressed proteins between C . cicadae -treated and non-treated (control) MHCC97H cells
| Spot No. 1 | Protein name | GenInfo identifier 2 | Protein score 3 | Expression quantity (×10 4 ) control 4 | Expression quantity (×10 4 ) C . cicadae | Expression change ( C . Cicadae /control) | P | pI 5 | Mr (kDa) 5 |
|---|---|---|---|---|---|---|---|---|---|
| 0111 |
14-3-3 Gamma In Complex With A Phosphoserine Peptide |
gi|82407948 |
76 |
33.0 ± 7.2 |
19.0 ± 5.3 |
0.58 |
0.0004 |
4.80 |
28.3 |
| 1217 |
microtubule-associated protein RP/EB family member 1 |
gi|6912494 |
219 |
10.0 ± 3.7 |
5.7 ± 2.2 |
0.57 |
0.026 |
5.02 |
30.1 |
| 1222 |
Chloride intracellular channel protein 1 |
gi|4588526 |
307 |
24.8 ± 8.4 |
9.3 ± 3.3 |
0.38 |
0.004112 |
5.02 |
27.2 |
| 1305 |
WD-40 repeat protein |
gi|4519417 |
221 |
42.3 ± 10.2 |
12.0 ± 4.1 |
0.28 |
0.001183 |
4.93 |
38.8 |
| 1427 |
Tubulin, beta 2C |
gi|23958133 |
188 |
3.6 ± 1.7 |
7.8 ± 4.2 |
2.17 |
0.025345 |
4.83 |
50.2 |
| 1514 |
Dynactin subunit 2 |
gi|22096346 |
436 |
9.8 ± 2.4 |
19.2 ± 5.8 |
1.97 |
0.000491 |
5.10 |
44.3 |
| 2303 |
thioredoxin-like protein |
gi|3646128 |
123 |
9.3 ± 5.4 |
3.4 ± 1.9 |
0.37 |
0.038567 |
5.25 |
37.8 |
| 2403 |
ACTB protein |
gi|15277503 |
444 |
101.7 ± 33.4 |
60.3 ± 18.3 |
0.59 |
0.001814 |
5.55 |
40.5 |
| 3413 |
N-myc downstream regulated gene 1 |
gi|119612570 |
105 |
9.0 ± 3.3 |
30.8 ± 11.8 |
3.44 |
0.00106 |
5.98 |
34.0 |
| 4102 |
heat shock protein beta-1 |
gi|4504517 |
368 |
18.1 ± 7.6 |
27.7 ± 10.6 |
1.53 |
0.026641 |
5.98 |
22.8 |
| 4504 |
xaa-Pro dipeptidase isoform 3 |
gi|260593665 |
193 |
8.7 ± 2.7 |
3.3 ± 1.2 |
0.38 |
0.027093 |
5.7 |
48.0 |
| 5101 |
Enoyl-CoA delta isomerase 1, mitochondrial |
gi|60593479 |
67 |
11.8 ± 7.8 |
5.8 ± 2.9 |
0.49 |
0.040511 |
6.00 |
28.9 |
| 5106 |
The Protein-Disulfide Isomerase Related Chaperone Erp29 |
gi|192987144 |
247 |
16.3 ± 6.8 |
9.3 ± 4.3 |
0.57 |
0.033331 |
7.07 |
27.2 |
| 5218 |
phosphatidylinositol transfer protein beta isoform |
gi|6912594 |
181 |
5.5 ± 1.9 |
8.5 ± 2.6 |
1.56 |
0.004165 |
6.41 |
31.8 |
| 5425 |
ectonucleoside triphosphate diphosphohydrolase 5, isoform |
gi|119601555 |
181 |
5.9 ± 2.1 |
2.3 ± 0.8 |
0.39 |
0.007848 |
5.74 |
45.7 |
| 6334 |
hnRNP 2H9B |
gi|7739445 |
127 |
31.1 ± 12.6 |
17.4 ± 6.5 |
0.56 |
0.023 |
6.76 |
31.5 |
| 6407 |
alpha-enolase isoform 1 |
gi|4503571 |
349 |
21.4 ± 7.5 |
34.8 ± 12.6 |
1.63 |
0.028265 |
7.01 |
47.5 |
| 6730 |
WD repeat-containing protein 1 isoform 1 |
gi|9257257 |
217 |
9.0 ± 4.6 |
13.9 ± 6.3 |
1.54 |
0.01346 |
6.17 |
66.8 |
| 7122 |
peroxiredoxin 1 |
gi|55959887 |
79 |
2.7 ± 1.0 |
0.5 ± 0.1 |
0.20 |
0.024662 |
6.41 |
19.1 |
| 7306 | mitotic checkpoint protein BUB3 isoform a | gi|4757880 | 147 | 37.1 ± 17.4 | 22.1 ± 10.1 | 0.60 | 0.045568 | 6.36 | 37.6 |
1Spot no.: automatically assigned by the PDQuest software.2GenInfo identifier: sequence identification number assigned by GenBank.
3Protein score: generated by the MS identification system.
4Expression Quantity were calculated by the PDQuest software, data expressed as mean ± SD.
5Mr and pI: relative molecular mass (Mr) and isoelectric point (pI) generated by the MS system.