TABLE 1.

Measured parameter values and calculated rate constants

The top section gives experimentally measured parameter values, extracted from the cited references, and rate constants for the kinase reactions in Reaction 1, calculated as explained under “Experimental Procedures,” for both the unphosphorylated (Ser-32) and the phosphorylated (Ser(P)-32) enzyme. The bottom section gives the same for the phosphatase reactions in Reaction 2. 1 unit = 1 μmol product/min.

	Parameter	Ser-32	Ser(P)-32	Units	Refs.
PFK-2	S0.5 ATP	150	150	μm	24
	S0.5 F6P	32	492	μm	21
	Vmax	129	44	milliunits/mg	21
	a1	7.86 × 10+2	2.70 × 10+2	m−1 s−1
	b1	1.18 × 10−1	4.04 × 10−2	s−1
	a2	7.36 × 10+6	1.64 × 10+5	m−1 s−1
	b2	4.71 × 10+2	1.62 × 10+2	s−1
	c1	2.36 × 10−1	8.09 × 10−2	s−1
	r1	2.36 × 10−1	8.09 × 10−2	s−1
FBPase-2	S0.5 F2,6BP	100	100	nm	57
	Vmax	86	177	milliunits/mg	21
	a3	2.00 × 10+7	2.00 × 10+7	m−1 s−1
	b3	3.84 × 100	3.68 × 100	s−1
	c2	1.56 × 10−1	3.23 × 10−1	s−1
	r2	1.56 × 10−1	3.23 × 10−1	s−1