TABLE 1.
Primer sequences for RT-PCR, RNAi, and overexpression
| Primer name | (5′ →3′) nucleotide sequence |
|---|---|
| β-actin F | Agtagccgccctggttgtagac |
| β-actin R | Ttctccatgtcgtcccagt |
| PLCG1-qRTF | Ccagtgctcgctccctacaac |
| PLCG1-qRTR | Ggcttatcaggagtctggtagttca |
| PLCG1-OEF1 | tactcacaattggatgaatccggtctgctataat |
| PLCG1-OER1 | tactcaggcgcgccgatttgctcgtgatgaaggatat |
| dsGFPT7F | gcgtaatacgactcactataggtggtcccaattctcgtggaac |
| dsGFPT7R | Gcgtaatacgactcactataggcttgaagttgaccttgatgcc |
| dsPLCG1T7F | gcgtaatacgactcactataggggttccgcacatttctgaggt |
| dsPLCG1T7R | gcgtaatacgactcactatagggaacgggattgtagttcgacg |
| dsErGPCRT7F | Gcgtaatacgactcactatagggttcatccttctaacggtggc |
| dsErGPCRT7R | Gcgtaatacgactcactatagggtcgcttcatcttcgctatct |
| ErGPCR-qRTF1 | Aaacggttcacctactacgc |
| ErGPCR-qRTR1 | Cgcttcatcttcgctatct |
| dsDopEcRT7F | Gcgtaatacgactcactataggtgaccaacgatgctggttac |
| dsDopEcRT7R | Gcgtaatacgactcactataggctgctctgatgaggggtagc |
| dsGαqT7F | Gcgtaatacgactcactataggtcggaggaggcgaaggag |
| dsGαqT7R | Gcgtaatacgactcactatagggtgttcgccgcagtcaaa |
| dsCDK10T7F | Gcgtaatacgactcactataggttgaacggcagcatagtgg |
| dsCDK10T7R | gcgtaatacgactcactataggagagtgacgacgcgaggag |
| DopEcR-qRTF1 | Tgacggaaagcaggttgag |
| DopEcR-qRTR1 | gaagccagcagaagacgaa |
| Gαq-qRTF1 | Ggcagttgcgaaaggac |
| Gαq-qRTR1 | Tctgagttggacggatt |
| EcRB1-qRTF1 | Aattgcccgtcagtacga |
| EcRB1-qRTR1 | Tgagcttctcattgagga |
| USP1-qRTF1 | Ggtcctgacagcaatgtt |
| USP1-qRTR1 | Ttccagctccagctgactgaag |
| HR3-qRTF1 | Tcaagcacctcaacagcagcccta |
| HR3-qRTR1 | Gactttgctgatgtcaccctccgc |
| BrZ2-qRTF1 | Ggtgactgtccttactgcggcat |
| BrZ2-qRTR1 | Ttaattcctttgaccatgact |