TABLE 2.

Primers used in this study

	Primer sequence	Underlined region
External primers
QcrALnew	CGGAAGGTTACTAGTAGGGGTGACTTAGAGGGGG	SpeI
QcrARnew	CCG GGATCC GTGTAATATCAAGCCGCTCG	BamHI
Internal primers
QcrAH102L-left	GTACACCCTAAAAGCTTACAAATTG	CAT → CTT (His → Leu)
QcrAH102L-right	CAATTTGTAAGCTTTTAGGGTGTAC	CAT → CTT (His → Leu)
QcrAH124L-left	CCGTAAAGGCATGGACAAAAGAATTTAT	CAT → CTT (His → Leu)
QcrAH124L-right	ATAAATTCTTTTGTCCATGCCTTTACGG	CAT → CTT (His → Leu)
QcrAC123S-left	CCGTAATGGGATGGACAAAAGAATTTAT	GCC-CCC (Cys → Ser)
QcrAC123S-right	ATAAATTCTTTTGTCCATCCCATTACGG	GCC-CCC (Cys → Ser)