FIGURE 1.

Growth analysis of wild type and nucleotide exchange factor deletion strains. A, serial dilutions of cells were plated onto rich (YPD) medium and incubated at the indicated temperatures. All other strains have the indicated genotypes. B, automated growth curves in liquid medium were generated as described under “Experimental Procedures.” WT, black; sse1Δ, blue; sse2Δ, gray; fes1Δ, red; snl1Δ, maroon; fes1Δ sse1Δ, yellow; fes1Δ sse2Δ, orange; fes1Δ snl1Δ, light purple; snl1Δ sse1Δ, light blue; snl1Δ sse2Δ, brown; fes1Δ snl1Δ sse1Δ, violet; fes1Δ snl1Δ sse2Δ, green.