TABLE 2.

Lag time values of HST3 mutant strains in the presence or absence of MMS

MMS was present immediately following dilution of saturated cultures to low cell density. Average lag time values and S.E. were calculated for three technical replicates.

Treatment	Strain	Average lag time (h) ± S.E.
No MMS	hst3Δ hst4Δ [pCEN HST3-TAP]	18.6 ± 0.2
	hst3Δ hst4Δ [pCEN HST3-T380A-TAP]	21.4 ± 0.4
	hst3Δ hst4Δ [pCEN HST3-T384A-TAP]	21.8 ± 0.7
	hst3Δ hst4Δ [pCEN HST3 2A-TAP]	21.7 ± 0.8
0.001% MMS	hst3Δ hst4Δ [pCEN HST3-TAP]	20.7 ± 0.4
	hst3Δ hst4Δ [pCEN HST3-T380A-TAP]	24.0 ± 0.2
	hst3Δ hst4Δ [pCEN HST3-T384A-TAP]	24.0 ± 0.7
	hst3Δ hst4Δ [pCEN HST3 2A-TAP]	24.1 ± 0.7
0.002% MMS	hst3Δ hst4Δ [pCEN HST3-TAP]	22.1 ± 0.1
	hst3Δ hst4Δ [pCEN HST3-T380A-TAP]	25.7 ± 0.7
	hst3Δ hst4Δ [pCEN-HST3-T384A-TAP]	26.8 ± 1.0
	hst3Δ hst4Δ [pCEN HST3 2A-TAP]	25.7 ± 1.2
0.004% MMS	hst3Δ hst4Δ [pCEN HST3-TAP]	24.4 ± 0.3
	hst3Δ hst4Δ [pCEN HST3-T380A-TAP]	27.8 ± 1.0
	hst3Δ hst4Δ [pCEN HST3 T384A-TAP]	28.7 ± 0.8
	hst3Δ hst4Δ [pCEN HST3 2A-TAP]	28.4 ± 1.2