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. 2014 Mar 19;289(19):13206–13218. doi: 10.1074/jbc.M113.522953

FIGURE 3.

FIGURE 3.

Localization studies using confocal microscopy and subcellular fractionation. A, subcellular localization of hPLSCR1-GFP. B, subcellular localization of ΔPRD-hPLSCR1-GFP. C, subcellular localization of hPLSCR2-GFP. D, subcellular localization of PRD-hPLSCR2-GFP. COS-7-transfected cells (green) were serum-starved to facilitate induction of apoptosis, and the subcellular localization was determined. Nuclear (N), cytoplasmic (CP), and PM distribution was determined using DAPI (blue) nuclear-specific dye and GFP (green). HEK cells were fractionated by differential centrifugation and probed by Western blot. E, subcellular fractionation of hPLSCR1-GFP expressing HEK293T cells; anti-PLSCR1 antibody was used to probe hPLSCR1-GFP localization. F, subcellular fractionation of hPLSCR2-GFP-expressing HEK cells; anti-PLSCR2 antibody was used to probe hPLSCR2-GFP localization. All the experiments were performed at least three times.