FIGURE 6.

Far UV-CD spectroscopy. A and B represent the secondary structure of hPLSCR1 in the presence of Ca²⁺ and Mg²⁺, respectively. C and D, ΔPRD-hPLSCR1 secondary structure in presence of Ca²⁺ and Mg²⁺, respectively. E and F, secondary structure of hPLSCR2 with Ca²⁺ and Mg²⁺, respectively. G and H, PRD-hPLSCR2 secondary structure in the presence of Ca²⁺ and Mg²⁺, respectively. Traces a, respective protein; traces b and c, respective protein in presence of 2 and 4 mm Ca²⁺ or Mg²⁺. The presence and absence of PRD have no major changes on the secondary structure content of PLSCRs. Results are representative of at least three set of experiments (p < 0.05).