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. 2014 Mar 19;289(19):13243–13258. doi: 10.1074/jbc.M114.557231

FIGURE 7.

FIGURE 7.

Dissolution of preaggregated TTR by mIgM 1802. A, reduced turbidity. Preaggregated TTR (7.1 μm) was incubated in the presence of hydrolytic mIgM 1802 (120 μg/ml, 0.67 μm), an equivalent non-hydrolytic IgM 1819 concentration, or diluent. mIgM 1802 treatment reduced the turbidity to the basal value observed for control non-aggregated TTR treated with mIgM 1802. Inset, photograph showing turbid misTTR suspension (white precipitates against black background) following incubation in diluent and misTTR dissolution after treatment with the hydrolytic IgM. B, reduced ThT fluorescence. Shown are ThT fluorescence values after treating preaggregated TTR with hydrolytic mIgM 1802, non-hydrolytic mIgM, or diluent for 120 h as in A. mIgM 1802 treatment reduced the ThT fluorescence value to the basal value observed for non-aggregated TTR treated with diluent. Data are corrected for ThT fluorescence of IgMs alone without TTR substrate (84 ± 5 fluorescence units (FU)). Error bars, S.D.