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. 2014 Mar 14;289(19):13284–13294. doi: 10.1074/jbc.M113.539726

FIGURE 1.

FIGURE 1.

The type II CRISPR-Cas system of S. thermophilus LMG18311. A, Coomassie Blue-stained SDS-polyacrylamide gel of Cas9, Cas9 D9A, Cas9 D599A, and Cas9 D9A,D599A. B, logo plot revealing the PAM for LMG18311 Cas9. The positions of the protospacer, PAM, and linker are indicated. C, schematic representation of the crRNA (green), tracrRNA (blue), and DNA target (black). The positions of the protospacer, PAM, and linker are indicated. The site at which the crRNA and tracrRNA are fused to generate the sgRNA is indicated with a dotted line.