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. 2014 Mar 14;289(19):13284–13294. doi: 10.1074/jbc.M113.539726

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — LMG18311 Cas9 and cognate sgRNA can provide resistance to plasmid transformation in E. coli. A, schematic representation of transformation assay. B, interference of plasmid transformation by LMG18311 Cas9 and sgRNA in E. coli cells. Transformation efficiency is expressed as cfu per 5 ng of plasmid DNA. Average values from at least three biological replicates are shown, with error bars representing 1 S.D. C, effect of mutation in the PAM sequence on plasmid transformation efficiency. D, effect of linker length on plasmid transformation efficiency. PS denotes the protospacer sequence.