Skip to main content
. 2014 Mar 20;289(19):13295–13307. doi: 10.1074/jbc.M114.550541

FIGURE 2.

FIGURE 2.

Thrombin dose- and time-dependently increases MMP-1 and MMP-9 mRNA and enzyme activity in amnion mesenchymal but not epithelial cells. Primary human amnion epithelial or mesenchymal cells were treated with thrombin for the indicated dose and time. A, amnion cells were treated with different doses of thrombin for 48 h and analyzed for MMP1 (a), MMP2 (b), or MMP9 (c) mRNA. A.U., arbitrary units. B, gelatin zymography of conditioned media from cells treated with various concentrations of thrombin for 48 h (a) or cell-free incubation of conditioned media with thrombin (4 units/ml, 36 nm) for 48 h (b). C, MMP-1 enzymatic activity in conditioned media from cells treated with different doses of thrombin for 48 h. D, thrombin (2 units/ml, 18 nm)-induced increases in MMP1 (a) and MMP9 (b) mRNA as a function of time. E, MMP-1 enzymatic activity in conditioned media from amnion mesenchymal cells treated with 2 units/ml (18 nm) of thrombin as a function of time. F, gelatin zymography of conditioned media (5 μg of protein) from amnion mesenchymal cells. Upper zymogram represents MMP-9; lower, MMP-2. Cells were stimulated with 2 units/ml thrombin (+) or medium only (−) for different time periods. In A and C–E error bars represent mean ± S.D., n = 3 in each group. B and F represent results from at least three experiments. *, p < 0.05; **, p < 0.01 compared with controls (0 units/ml vehicle, A and C), 0 h (D), or 4 h thrombin treatment (E).