FIGURE 3.

Thrombin increases COX2 mRNA and PGE₂ synthesis in primary amnion mesenchymal cells. Primary amnion epithelial or mesenchymal cells were treated for the indicated dose or time. A, COX2 mRNA levels in amnion cells treated with 4 units/ml (36 nm) of thrombin × 24 h. A.U., arbitrary units. B, PGE₂ levels in conditioned media. Amnion cells were treated with 4 units/ml (36 nm) thrombin ×48 h. C, COX2 mRNA in amnion mesenchymal cells treated with control (medium only) or thrombin (4 units/ml, 18 nm) as a function of time. D, PGE₂ in conditioned media from amnion mesenchymal cells treated with thrombin (4 units/ml, 36 nm) for the indicated times. n = 3 in each group. *, p < 0.05; **, p < 0.01 compared with control (0 units/ml, A and B), 0 h (C), or 10 h thrombin treatment (D).