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. 2014 Mar 20;289(19):13295–13307. doi: 10.1074/jbc.M114.550541

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Comparison of pThr and rThr and endotoxin removal on MMP9, MMP1, and COX2 gene expression in amnion mesenchymal cells. A and B, cells were treated with various doses of pThr (0.5 (4.5 nm) to 4 (36 nm) units/ml or rThr (4 units/ml), and MMP1 (A) or MMP9 (B) mRNA levels were quantified after 48 h. *, p < 0.01 compared with control. A.U., arbitrary units. C and D, effect of endotoxin removal on pThr-induced MMP1 (C) and COX2 (D) mRNA. E, silver-stained gel of purified rThr and pThr. Thrombin was applied at 100 and 300 ng/lane as indicated. **, p < 0.01.