FIGURE 1.

Meprin A distribution in kidneys of mice subjected to ischemia-reperfusion or cisplatin injury. A–D, double-immunofluorescence of meprin A (red) and Na/K-ATPase (green) in kidney sections of control mice (A and C) and mice subjected to IR (24 h of reperfusion after 45 min of ischemia) (B) or cisplatin injury (D). Kidney tissue was fixed in phosphate-buffered 4% formalin and paraffin-embedded. Tissue sections (8 μm) were incubated with goat polyclonal anti-meprin-β and rabbit polyclonal anti-Na⁺/K⁺-ATPase antibodies overnight. After washing with PBS, slices were incubated with secondary antibodies from Molecular Probes (donkey anti-goat Alexa Fluor 594 (red) and donkey anti-rabbit Alexa Fluor 488 (green). Nuclei were stained with DAPI. Epifluorescence pictures were recorded on an Olympus (IX51, IX71, or BX51) microscope.