TABLE 1.

Melting temperature predictions for hybrids between recombinant RNAs and mutated or WT mtDNA regions

RNA	Insert (bp)	Tm for mutated DNA	Tm for WT DNAa	ΔTm
FD16L	16	48.4 °C	40.2 °C	8.2 °C
FD16H	16	48.4 °C	35.0 °C	13.4 °C
FD20L	20	54.9 °C	49.3 °C	5.6 °C
FD20H	20	52.8 °C	42.9 °C	9.9 °C
FD25L	25	59.0 °C	54.9 °C	4.1 °C
FD25H	25	59.0 °C	52.2 °C	6.8 °C

^a Shown are values predicted by IDT Sci-Tools OligoAnalyser software (version 3.1) for mismatch containing DNA-DNA hybrids. ΔT_m, predicted decrease of T_m for wild type mtDNA compared with mutated mtDNA.