FIGURE 4.
Characterization of clathrin-independent endocytosis inhibitors in MDCKI-NCC cells. Internalization assay of LacCer (BODIPY® FL C5-lactosylceramide complexed to BSA) (marker for clathrin-independent endocytosis) in MDCKI-NCC cells grown on plastic. A, 30 μm PitStop2 has no significant effect on the internalization of LacCer. B, internalization of LacCer after 15 and 30 min is significantly reduced following 25 μg/ml nystatin treatment. C, LacCer endocytosis is significantly inhibited at all time points examined with 20 mm MβCD. Data are means ± S.E. (n = 3). * represents significant change compared with control treatment at same time point.