FIGURE 1.
Soluble full-length chemerin (s-Chem(21–157)) has higher potency than soluble chemerin(145–157) at both human (a) and mouse (b) CMKLR1. s-Chem(145–157) and s-Chem(21–157) were purchased commercially. HEK293 cells were transiently transfected with cDNAs encoding (i) human or mouse CMKLR1, (ii) a SRE5x-Luc-PEST reporter gene, (iii) a Gαq5i66V chimera, and (iv) a β-galactosidase control. Twenty-four hours after transfection cells were stimulated with ligand for 4 h and luciferase activity was determined as described under “Experimental Procedures.” Luciferase activity was normalized relative to the maximal value observed using saturating concentrations of the corresponding soluble ligand (=100%). Data points represent the mean ± S.E. from at least three independent experiments, each performed in triplicate.