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. 2014 Mar 21;289(19):13385–13396. doi: 10.1074/jbc.M113.522680

FIGURE 2.

FIGURE 2.

Tethered chemerin 9 (t-Chem(149–157)) activity exceeds that of full-length chemerin (t-Chem(21–157)) at the human receptor (a). In contrast, activation of the mouse receptor by these two constructs is comparable (b). Pre-prochemerin (t-Chem(1–163)) and the control (tGalanin) lack activity at both human and mouse receptors. HEK293 cells were transiently transfected with cDNAs encoding (i) indicated tethered ligand, (ii) human or mouse CMKLR1, (iii) a SRE5x-Luc-PEST reporter gene, (iv) a Gαq5i66V chimera, and (v) a β-galactosidase control. Twenty-four hours after transfection, luciferase activity was determined as described under “Experimental Procedures.” Luciferase activity was normalized relative to the maximal value observed using t-Chem(21–157) cDNA (=100%). Data points represent the mean ± S.E. from at least three independent experiments, each performed in triplicate. Comparison of t-Chem(21–157) to t-Chem(149–157) is shown: ***, p < 0.001.