TABLE 1.

Chemical structure, purity, and molecular weight of synthesized peptides

^a Purity was determined by analytical RP-HPLC (Vydac C18, 5 μm, 4 × 250 mm) and a binary solvent system (A: H₂O/CH₃CN/TFA (99/1/0.1); B: CH₃CN/H₂O/TFA (90/10/0.07)) with a linear gradient of solvent B over 20 min. Flow rate was at 1 ml/min and elution was monitored at by absorbance at 230 nm.

^b Expected molecular weights were calculated using Peptide mass calculator version 3.2.

^c Observed molecular weights were determined by MALDI-TOF MS in positive reflectron mode using α-cyano-4-hydroxycinnamic acid as the matrix.

^d KGG spacer coupled to the N terminus of the peptide before pegylation.

^e GG spacer coupled to the N terminus of the peptide before pegylation.