TABLE 4.
Summary of the HPLC and MS analysis of oligosaccharide substrates
| Compound | Abbreviated structures | Purity | Amount prepared | Molecular weight |
|---|---|---|---|---|
| % | mg | |||
| 1 | GlcNS-GlcA-GlcNS-GlcA-PNP | 98 | 1.5 | 973.6 ± 0.0 (973.8) |
| 2 | GlcA-GlcNS-GlcA-GlcNS-GlcA-PNP | 97 | 2.0 | 1149.8 ± 0.4 (1149.9) |
| 3 | GlcNAc-GlcA-GlcNS-GlcA-GlcNS-GlcA-PNP | 98 | 12.5 | 1353.4 ± 0.2 (1353.1) |
| 4 | GlcNAc-GlcA-GlcNS-IdoA-GlcNS-GlcA-PNP | 96 | 0.7 | 1353.0 ± 0.4 (1353.1) |
| 5 | GlcNAc-GlcA-GlcNH2-GlcA-GlcNS-GlcA-PNP | 99 | 2.5 | 1272.9 ± 0.2 (1273.0) |
| 6 | GlcNAc-GlcA-GlcNAc-GlcA-GlcNS-GlcA-PNP | 99 | 8.5 | 1315.0 ± 0.4 (1315.1) |
| 7 | GlcNAc-GlcA-GlcNS-GlcA-GlcNAc-GlcA-PNP | 99 | 10 | 1315.0 ± 0.5 (1315.1) |
| 8 | GlcNAc6S-GlcA-GlcNS6S-GlcA-GlcNS6S-GlcA-PNP | 96 | 0.3 | 1593.1 ± 0.3 (1593.3) |
| 9 | GlcNAc-GlcA-GlcNS-GlcA-GlcNS6S-GlcA-PNP | 97 | 5.0 | 1433.2 ± 0.3 (1433.2) |
| 10 | GlcNAc-GlcA-GlcNS-GlcA-GlcNAc6S-GlcA-PNP | 98 | 6.0 | 1395.3 ± 0.6 (1395.2) |
| 11 | GlcA-GlcNAc-GlcA-GlcNS-GlcA-GlcNS-GlcA-PNP | 98 | 25 | 1529.4 ± 0.6 (1529.3) |
| 12a | GlcA-GlcNAc-GlcA-GlcNS-GlcA-GlcNS-GlcA-PNP and GlcA-GlcNAc-GlcA-GlcNS-IdoA-GlcNS-GlcA-PNP | 52 and 48 | 60 | 1529.4 ± 0.9 (1529.3) |
| 13 | GlcNAc-GlcA-GlcNS-GlcA-GlcNS-IdoA2S-GlcNS-GlcA-PNP | 99 | 5.0 | 1850.0 ± 0.1 (1850.5) |
| 14 | GlcNAc-GlcA-GlcNS-IdoA-GlcNS-IdoA2S-GlcNS-GlcA-PNP | 98 | 0.5 | 1850.4 ± 0.5 (1850.5) |
a Compound 12 was used to obtain the co-crystal structure with 2-OST.