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Summary of mutant analysis using both GlcA-containing and IdoA-containing substrates
| Name of mutants | Reactivity to compound 3 (GlcA-containing substrate) | Reactivity to compound 4 (IdoA-containing substrate) |
|---|---|---|
| % | % | |
| 2OST WT | 100 | 100 |
| 2OST Y94A | 23.0 | 35.7 |
| 2OST H106A | 16.8 | 27.7 |
| 2OST R184A | 10.1 | 8.3 |
| 2OST R189A | 81.7 | 2.4 |
| 2OST K354A | 82.0 | 101.2 |
