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. 2014 Mar 20;289(19):13531–13542. doi: 10.1074/jbc.M113.525287

FIGURE 4.

FIGURE 4.

Agarose overlay reduces HA release into the medium. A, the HA concentration measured by ELISA assay was reduced in culture media from HSF, AMSC, and AMEC following agarose overlay for 5 days in both serum-free and FBS-containing conditions when compared with that without agarose overlay. B, phase-contrast micrographs of HSF, AMSC, and AMEC cultured in both FBS-free and FBS-containing conditions with or without agarose overlay for 5 days. Scale bar = 50 μm.