Agarose overlay reduces HA release into the medium.
A, the HA concentration measured by ELISA assay was reduced in culture media from HSF, AMSC, and AMEC following agarose overlay for 5 days in both serum-free and FBS-containing conditions when compared with that without agarose overlay. B, phase-contrast micrographs of HSF, AMSC, and AMEC cultured in both FBS-free and FBS-containing conditions with or without agarose overlay for 5 days. Scale bar = 50 μm.