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. 2014 Mar 27;289(19):13554–13564. doi: 10.1074/jbc.M114.550731

FIGURE 2.

FIGURE 2.

LysM-VCL osteoclasts differentiate into osteoclasts normally. A, WT (Cre-) and LysM-VCL BMMs were cultured in M-CSF and RANKL with time. Osteoclast differentiation markers were determined by immunoblot. β-Actin served as a loading control. B and C, WT and LysM-VCL BMMs were cultured in M-CSF and RANKL for 3 or 4 days. B, the cells were stained for TRAP activity. Scale bars, 200 μm. C, quantitation of TRAP-expressing multinucleated cells (MNCs) (more than 3 nuclei/cell) generated from WT and LysM-VCL BMMs. D, WT and LysM-VCL BMMs were cultured in M-CSF and RANKL for 4 days. The diameter of MNCs was measured. ***, p < 0.001. Error bars, S.D.