CtsK-VCL mice are osteopetrotic.
A, μCT image of distal femur of WT and CtsK-VCL mice at 9 weeks of age. B, μCT determination of BV/TV, trabecular number, trabecular thickness, and trabecular separation of distal femoral metaphysis of WT and CtsK-VCL mice at 9 weeks. C, TRAP-stained histological sections of proximal tibia of WT and CtsK-VCL mice. Black boxes indicate the region of magnification shown in the bottom panels. D, BV/TV, total number of osteoclasts normalized to bone surface (Oc.N/BS), and the percentage of bone surface to which osteoclasts are juxtaposed (Oc.S/BS) in C were histomorphometrically determined. *, p < 0.05; **, p < 0.01. Scale bars, 1 mm (A), 500 μm (C, top panels), 50 μm (C, bottom panels). Error bars, S.D.