Figure 5.

Expression of anx1 paralogs. (A—C) Lateral views of 70% epiboly stage embryos revealing enveloping layer expression of anx1a (A), anx1b (B), and anx1b (C). (D) anx1a expression is markedly reduced by the 12-somite stage and then reappears in the periderm (E) at 24 hpf. (F) Periderm expression of anx1c at 24 hpf. Arrowhead indicates a domain of anx1c expression not found with anx1a and anx1b. (G) A higher magnification dorsal view of the anx1c unique expression domain. A ventral view(H) and sagittal section (I) of 80-hpf embryos showing anx1a expression in the gills, epithelium, fin buds, and the cells of the swim bladder (arrowhead). (J) At 80 hpf, anx1c was expressed predominately in the epithelium. (K) Embryonic and adult expression of anx1a determined by Northern analysis. Total RNA was isolated from zebrafish embryos at selected stages from the one-cell stage to adult. Scale bar, 200 μm.