Figure 6.

Expression of anx2 genes. (A) Dorsal view of a 100% epiboly stage embryo revealing both notochord and enveloping layer expression (arrowhead) of anx2a. (B) Higher magnification view of embryo in A, highlighting enveloping layer expression. (C) A lateral view of notochord expression of anx2a throughout the entire anterior to posterior axis; (D) a higher power lateral view of the same embryo. (E) A lateral view of a 21-somite embryo with only posterior anx2a expression remaining. (F) At 24 hpf, anx2a expression is predominately in the periderm not notochord and then later in the skin (data not shown). (G) anx2b expression in the intestine at 72 hpf (arrowhead). Sagittal sections through both the proximal (H) and distal (I) intestine indicate anx2b expression in the apical brush border of the intestinal epithelium (arrowhead).