Figure 7.

Expression of anx4. (A) Dorsal view of a four-somite embryo revealing anx4 transcripts in the floor plate (arrowhead) and in two lateral stripes (arrow) corresponding to pronephric duct precursor cells. Lateral views of a 21-somite (B) and a 24-hpf (C) embryo with prominent anx4 expression in the floor plate, hypochord, and pronephros. (D) Dorsal view at 24 hpf also shows anx4 expression in the otolith, glomerulus (midline arrowhead), pronephric tubulues, and pronephric ducts. (E) A similar dorsal view illustrating pax2 transcripts in the otolith, pronephric tubulues, and pronephric ducts but not the glomerulus. Hematopoietic precursors were simultaneously visualized in cross sections using one riboprobe for draculin transcripts (red) and another for anx4 transcripts (blue) at both the 20-somite (F) and (G) 24-hpf stages. (H) A lateral view at 24 hpf also localizes anx4 expression to floor plate (arrowhead), hypochord (arrow), and pronephros. Cross sections of 3-hpf larvae reveal anx4 expression predominately in the gall bladder (I) and liver (J). (A—E) Bar, 200 μm; (F—J) bar, 100 μm.