Table 2.

Pairwise Comparison of ZNF Genes in Selected Clades, Using the Ratio of Nonsynonymous Differences per Nonsynonymous Site (d_N) Over Synonymous Differences per Synonymous Site (d_s)

Section A: Comparisons involving all amino acid positions in finger motifs
Group I											Group II
	ZNF226	ZNF234	Zfp61	ZNF225	ZNF230	ZNF223	ZNF155	ZNF284	ZNF221	ZNF222		Zfp112
ZNF234	0.301 ×										ZNF228	0.102 ×
Zfp61	0.166 ×	0.165 ×
ZNF225	0.383 ×	0.327 ×	0.224 ×
ZNF230	0.442 ×	0.402 ×	0.219 ×	0.363 ×
ZNF223	0.428 ×	0.445 ×	0.197 ×	0.414 ×	0.364 ×						Group III
ZNF155	0.378 ×	0.448 ×	0.203 ×	0.388 ×	0.398 ×	0.352 ×						Zfp94
ZNF284	0.438 ×	0.471 ×	0.232 ×	0.379 ×	0.461 ×	0.51 ×	0.465 ×				ZNF45	0.122 ×
ZNF221	0.367 ×	0.383 ×	0.207 ×	0.471 ×	0.532 ×	0.552 ×	0.604 ×	0.486 ×
ZNF222	0.453 ×	0.485 ×	0.181 ×	0.444 ×	0.385 ×	0.329 ×	0.471 ×	0.541 ×	0.506 ×
ZNF224	0.359 ×	0.358 ×	0.184 ×	0.362 ×	0.461 ×	0.55 ×	0.368 ×	0.451 ×	0.253 ×	0.426 ×
Group IV											Group V & ZNF283, ZNF404
	Zfp235	ZNF235	Zfp93	Zfp108	Zfp109	Zfp114						Zfp180	ZNF404	ZNF283
ZNF235	0.024 ×										ZNF404	n/c
Zfp93	0.221 ×	0.047 ×									ZNF283	n/c	0.578 ×
Zfp108	0.234 ×	0.053 ×	0.298 ×								ZNF180	n/c	0.308 ×	0.25
Zfp109	0.288 ×	0.09 ×	0.335 ×	0.324 ×
Zfp114	0.283 ×	0.07 ×	0.361 ×	0.331 ×	0.439 ×
Zfp111	0.314 ×	0.106 ×	0.363 ×	0.37 ×	0.297 ×	0.429 ×
Section B: Comparisons involving subset of amino acid positions critical to DNA sequence recognition
Group I											Group II
	ZNF226	ZNF234	Zfp61	ZNF225	ZNF230	ZNF233	ZNF155	ZNF284	ZNF221	ZNF222		Zfp112
ZNF234	0.491										ZNF228	0.145 ×
Zfp61	0.177 ×	0.232 ×
ZNF225	1.608	1.068	1.608
ZNF230	2.693*	1.258	1.893	1.21
ZNF223	2.026*	1.527	2.045	1.109	1.108						Group III
ZNF155	1.123	1.007	1.806	1.19	1.153	0.967						Zfp94
ZNF284	1.626*	1.068	1.462	0.74	2.324*	1.312	0.705				ZNF45	0.367
ZNF221	1.603	1.897*	n/c	1.16	1.478	1.783	1.074	0.852
ZNF222	2.088*	1.515	1.582	0.941	1.768	0.477	0.839	1.642	1.258
ZNF224	1.206	1.196	1.18	0.956	1.771	1.295	0.878	0.932	0.559	1.406
Group IV											Group V & ZNF283, ZNF404
	Zfp235	ZNF235	Zfp93	Zfp108	Zfp109	Zfp114						Zfp180	ZNF404	ZNF283
ZNF235	0 ×										ZNF404	n/c
Zfp93	0.502	0.165 ×									ZNF283	n/c	1.239
Zfp108	0.543	0.183	0.581								ZNF180	n/c	2.36*	1.461
Zfp109	0.823	0.275 ×	0.906	0.867
Zfp114	0.418	n/c	0.831	0.944	4.183*
Zfp111	0.468	0.293	0.776	0.615	0.649	0.383

× =significant evidence for purifying selection or ^* = positive selection, in results of separate one-tailed Z-tests on the difference between nonsynonymous differences per nonsynonymous site and synonymous differences per synonymous site, using a bootstrap calculation of variance (values not shown); n/c = proportion of synonymous differences too high to be adjusted by Juke-Cantor (1969) correction.