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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Nov 7;351(4):1054–1059. doi: 10.1016/j.bbrc.2006.10.168

Figure 3.

Figure 3

Clock subcellular localization and phenylephrine treatment. Panels A and B show immunostaining for Clock protein in untreated myocytes and those treated with 10 μM phenylephrine for 48 hours respectively. Panel C shows quantitation of Western blotting results of Clock protein distribution in subcellular fractions with and without 10μM phenylephrine treatment for 48 hours. Phenylephrine treatment results in a significant translocation of Clock from the membrane to the nuclear fraction. P<0.01, n=3 cultures. Panel D shows a Western blot of total Clock protein in untreated myocytes and following 48 hours of phenylephrine treatment. Clock protein increased about 3 fold following the drug treatment. P<0.01, n=3 cultures.