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. 2014 May 7;12:114. doi: 10.1186/1479-5876-12-114

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Copyright © 2014 Wen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Identification of multi-potential differentiation of MSCs and observation of MSCs compounded with fibrin glue. Cells were cultured and induced to differentiate into osteoblasts indicated by staining of Alizarin Red (A) and NBT-BCIP (B), chondroblasts by Alcian blue (C) and adipocytes by Oil Red O (D). (E) After fibrin glue-compounding within one week, normal cell shape under bright field and green fluorescence resulting from Ad-HGF transfection indicated the cell activity. (F) The morphologies of cells on the surface of and inside the fibrin glue were observed with the scanning electric microscopy.