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. 2014 May 7;12:114. doi: 10.1186/1479-5876-12-114

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Copyright © 2014 Wen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Proliferation, HGF expression and osteoblast differentiation of MSCs compounded with fibrin glue. Cell proliferation and viability were assayed using EdU incorporation (A, B) and CCK-8 method (C). HGF expression was assayed using ELISA (D). Osteoblast differentiation of MSCs compounded with or without fibrin glue was assayed using ALP staining (E) and ARS staining (F, G) after treatment with osteogenic culture medium.