Fig. 1.

Molecular characterization and apex stage development of the mvp mutant. (a) Total RNA was extracted from whole aerial parts and analysed by RT-PCR. Plants were acclimated at 4 ºC for 7 d under LD conditions. Each replicate (R1, R2, and R3) was obtained from three control wild-type plants (WT) or from five mutant plants (mvp). Relative expression level of gene markers of wild-type and mvp-mutant plants was analysed by RT-PCR to validate the mvp mutant. The 18S TaRNA was used for load control. The experiments were repeated three times and a representative result is shown. (b) Apex stage development of wild-type (WT) and mvp-mutant plants (mvp) used for molecular characterization and microarray analysis. The arrow indicates the double ridge and the scale bar is indicated.