Fig. 3.

Relative expression level of several selected genes in mvp-mutant and wild-type control plants analysed by RT-PCR and PCR experiments. (a) Validation of TmUnG and TmCir expression by RT-PCR. The RNA samples are the same as those used in Fig. 1 and the microarray experiment. The 18S TaRNA was used as load control. (b) Genomic DNA was extracted from the same plant samples used for the microarray analysis and were tested for PCR amplification to analyse the possible deletion of the two most repressed genes from the array analysis (TmUnG repressed 114 times and TmCir repressed 169 times). The 18S DNA is used as control. The experiments were repeated with 3 different biological replicates with the same result. Each replicate (R1, R2, and R3) was obtained from five mutants plants (mvp) and from three control wild-type plants.