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. 2014 Mar 28;65(9):2271–2286. doi: 10.1093/jxb/eru102

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Relative expression level of TaVRN1 and TaFT1 and MeJA content during vernalization in hexaploid winter wheat seedlings analysed by qRT-PCR and HPLC/MS. Two weeks after germination at 20 ºC under LD conditions, non-vernalized winter wheat (cv Norstar) plants were vernalized under SD conditions at 4 ºC for 63 d and deacclimated for 14 d at 20 ºC under LD conditions. The aerial part was sampled around 4h after the beginning of the daylight period. The expression level of TaVRN-A1 (panel a) and TaFT-A1 (panel b) are expressed relative to the non-vernalized point (Ctrl-0). Data represent the mean ± SEM from three biological replicates. The expression levels are normalized with the TaRNA 18S. Plant samples from the same experiment were used to measure the MeJA content by HPLC / MS (panel c). Data represent the mean ± SEM from three biological replicates. AC-35D: cold-acclimated for 35 d; AC-63D: cold-acclimated for 63 d; DA-14D; plants cold-acclimated for 63 d and deacclimated under favourable growth conditions (LD and 20 ºC).