Fig. 7.

Relative expression level of FUL2 and FUL3 analysed by qRT-PCR. (a, b) mvp-mutant and wild-type einkorn wheat plants. Plants were acclimated at 4 ºC for 7 d under LD conditions. The data represent the mean obtained from three biological replicates each using three control wild-type plants (WT) or five mvp-mutant plants (mvp). (c, d) During vernalization and de-acclimation conditions in hexaploid winter wheat seedlings (cv Norstar). After 2 weeks of germination at 20 ºC under LD conditions, non-vernalized winter wheat plants were vernalized under SD conditions at 4 ºC for 63 d and de-acclimated for 14 d at 20 ºC under LD conditions. The expression level of TaFUL2 (panel c) and TaFUL3 (panel d) are expressed relative to the non-vernalized plants (Ctrl-0). The aerial part was sampled around 4h after the beginning of the daylight period. (e, f) Effect of MeJA treatment in hexaploid spring wheat seedlings (Manitou). The expression level of TaFUL2 (panel e) and TaFUL3 (panel f) are expressed relative to the non-treated plants (Ctrl). Three weeks after germination at 20 ºC under LD conditions, control spring wheat (cv Manitou) plants (sprayed with 0.1% tween 20 solution only: Ctrl) were grown under LD conditions at 20 ºC for two weeks. Treated plants were sprayed with 150 μM MeJA dissolved in 0.1% tween solution every day for 2 weeks under the same growth conditions. Relative transcript abundance was calculated and normalized with respect to 18S TaRNA for the qRT-PCR experiment. Data represent the mean ± SEM from three biological replicates.