Table 2.
mvp wheat plant genes validated by qRT-PCR and their fold change on the microarray
| Affymetrix Probeset IDs |
GenBank Accession | UniProt and NCBI description | Fold change |
|---|---|---|---|
| Ta.3976.2.S1_x_at | CA679100 | Triticum aestivum flavanone 3-hydroxylase mRNA, partial cds | 6.713 |
| Ta.27327.1.S1_x_at | BT009360 | Pathogenesis-related 1a n=1 Tax=Triticum monococcum RepID=Q3S4I4_TRIMO | 5.467 |
| Ta.959.1.S1_at | CA721939 | Thaumatin-like protein n=3 Tax=Triticum RepID=Q41584_WHEAT | 5.147 |
| Ta.221.1.S1_at | AF112963 | Chitinase II n=1 Tax=Triticum aestivum RepID=Q9XEN3_WHEAT | 4.725 |
| TaAffx.104812.1.S1_s_at | BJ223744 | Lipoxygenase 2.1, chloroplastic n=1 Tax=Hordeum vulgare RepID=LOX21_HORVU | 4.031 |
| Ta.10215.1.S1_at | AY679115 | Triticum aestivum gigantean 3 (TaGI3) mRNA, complete cds | –0.035 |
| Ta.11017.1_A1_at | B4ERX7 | Triticum aestivum O-methyltransferase | –0.447 |
| Ta.30640.1.S1_at | CD861747 | Triticum aestivum flowering locus T mRNA, complete cds or VRN3 | –3.250 |
| Ta.28005.1.A1_at | CD862101 | Phytochrome C (Fragment) n=1 Tax=Hordeum vulgare RepID=Q945T7_HORVU | –16.267 |
| TaAffx.143995.17.S1_s_at | AY188331 | MADS box transcription factor n=15 Tax=Triticeae RepID=O82128_WHEAT | –108.744 |
| TaAffx.85922.1.S1_s_at | CA618396 | Putative uncharacterized protein Sb01g007930 n=2 Tax=Poaceae RepID=C5X0B2_SORBI (UnG) | –114.866 |
| Ta.29481.1.S1_at | CK194207 | Circumsporozoite protein n=2 Tax=Andropogoneae RepID=B6TF33_MAIZE | –169.064 |
The annotation is made according to Affymetrix Gene Chip® wheat genome array of the 12 probeset IDs used in RT-PCR and qRT-PCR experiments to validate microarray expression profiling analysis complemented with BLAST results showing the Genbank accession number, UniProt or NCBI description and is presented in decreasing order of differential expression of ≥2-fold and ≤–2-fold cut off.