Table 1.
Differentially accumulated transcripts between TR185 and wild type, annotated as related to N-acquisition pathwayAverage Affymetrix GeneChip normalized expression values across three biological replicates for the mutant TR185 and the wild type in high and low N conditions. G, N, and G×N indicate transcripts responsive to genotype, nitrate, and genotype×nitrate effect, respectively (adjusted P-values <0.05). G* indicates a transcript responsive to G effect only under HN condition. N* indicates a transcript responsive to N effect only for the wild type. G and G indicate upregulation and downregulation, respectively, in TR185 compared with the wild type.
| Target identifier | Annotation | MHN | MLN | WTHN | WTLN | Effect |
|---|---|---|---|---|---|---|
| Putative nitrate transporters | ||||||
| Mtr.44342.1.s1_at | NRT2 Nitrate transporter | 5.13 | 4.15 | 2.76 | 3.19 | G |
| Mtr.47690.1.s1_s_at | NRT1 Nitrate transporter | 7.10 | 6.97 | 6.19 | 5.81 | G |
| Ammonium transporters | ||||||
| Mtr.1706.1.s1_s_at | AMT1 transporter | 7.78 | 8.54 | 6.75 | 7.43 | G |
| Mtr.25576.1.S1_at | AMT2 transporter | 7.60 | 7.11 | 5.89 | 5.21 | G |
| Ammonium assimilation | ||||||
| Mtr.33664.1.s1_at | Ferredoxin-dependent glutamate synthase | 4.53 | 3.60 | 3.58 | 4.55 | G×N |
| Mtr.42847.1.s1_at | Pyrroline-5-carboxylate synthetase | 8.80 | 9.58 | 9.83 | 8.66 | G×N |
| Mtr.43170.1.s1_s_at | Glutamate deshydrogenase | 11.81 | 12.07 | 11.04 | 10.90 | G |
| Mtr.25985.1.s1_at | Putative l-asparagine amidohydrolase | 14.05 | 13.66 | 12.57 | 12.76 | G |
| Msa.1503.1.s1_at | Putative l-asparagine amidohydrolase | 12.92 | 12.80 | 11.40 | 11.72 | G |
| Mtr.39486.1.s1_at | Dihydrodipicolinate synthase | 3.27 | 4.48 | 6.19 | 6.77 | G |
| Putative amino acid transporters | ||||||
| Mtr.12447.1.s1_at | Proline transporter | 9.30 | 9.68 | 10.30 | 10.63 | G |
| Mtr.31521.1.s1_at | Lysine histidine transporter | 8.03 | 8.52 | 6.77 | 6.99 | G |
| Mtr.38963.1.s1_at | Amino acid transporter | 5.82 | 6.28 | 4.74 | 5.43 | G |
| Putative peptide transporters | ||||||
| Mtr.7014.1.s1_at | NRT1 peptide transporter | 9.21 | 9.63 | 8.22 | 8.62 | G |
| Mtr.48790.1.s1_at | Proton-dependent oligopeptide transporter | 5.15 | 4.42 | 3.92 | 3.50 | G |
| Organic acid metabolism | ||||||
| Mtr.32662.1.s1_at | beta-Amylase | 7.24 | 7.39 | 6.25 | 6.30 | G |
| Mtr.13958.1.s1_at | beta-Amylase | 6.87 | 7.13 | 5.82 | 5.73 | G |
| Mtr.12555.1.s1_at | Starch phosphorylase | 5.15 | 5.07 | 3.41 | 3.97 | G |
| Mtr.23663.1.S1_at | Phosphoenolpyruvate carboxylase kinase | 7.45 | 6.57 | 5.94 | 7.53 | N* G×N |
| Sugar transporters | ||||||
| Mtr.12578.1.s1_at | Malate transporter | 10.85 | 11.33 | 9.95 | 10.32 | G |
| Mtr.33536.1.s1_at | Sugar transporter | 5.47 | 4.89 | 4.10 | 5.24 | G* |
| Mtr.19796.1.s1_at | Glucose transporter | 7.65 | 8.58 | 8.97 | 9.37 | G |
| Msa.905.1.S1_at | Leghaemoglobin MtLb1 | 6.51 | 6.64 | 6.04 | 4.37 | G |