Table 3.
Differentially accumulated transcripts between TR185 and wild type, annotated as related to phenylpropanoid pathwayAverage Affymetrix GeneChip normalized expression values across three biological replicates for the mutant TR185 and the wild type in high and low N conditions. G, N, and G×N indicate transcripts responsive to genotype, nitrate, and genotype×nitrate effect, respectively (adjusted P-values <0.05). G and G indicate upregulation and downregulation, respectively, in TR185 compared with the wild type.
| Target identifier | Annotation | MHN | MLN | WTHN | WTLN | Effect |
|---|---|---|---|---|---|---|
| Phenylpropanoid pathway | ||||||
| Mtr.40166.1.s1_s_at | Phenylalanine ammonia-lyase | 10.39 | 11.19 | 9.46 | 10.18 | G |
| Mtr.12988.1.s1_at | 4-Coumarate-CoA ligase | 6.64 | 6.68 | 7.54 | 7.71 | G |
| Lignin pathway | ||||||
| Mtr.20618.1.s1_s_at | Transferase family protein (HCT) | 5.94 | 7.29 | 7.58 | 7.86 | G |
| Mtr.4076.1.s1_at | Caffeoyl-CoA 3-O-methyltransferase | 5.45 | 5.80 | 6.22 | 6.95 | G |
| Mtr.40942.1.s1_at | Caffeoyl-CoA 3-O-methyltransferase | 4.26 | 4.52 | 5.36 | 5.39 | G |
| Mtr.10331.1.s1_at | Isoflavone-O- methyltransferase | 9.49 | 9.80 | 10.44 | 10.79 | G |
| Mtr.51214.1.s1_at | O-Methyltransferase | 3.21 | 3.42 | 3.97 | 4.75 | G |
| Flavonoid pathway | ||||||
| Mtr.49423.1.s1_at | Chalcone synthase | 5.01 | 4.81 | 4.15 | 3.22 | G |
| Dihydroflavonol pathway | ||||||
| Mtr.20354.1.S1_at | UDP-Glucose flavonol 3-O-glucosyltransferase | 7.81 | 7.03 | 6.09 | 5.46 | G |
| Mtr.28721.1.s1_at | UDP-Glucose flavonol 3-O-glucosyltransferase | 6.46 | 6.06 | 5.32 | 5.25 | G |
| Mtr.44246.1.S1_at | UDP-Glucose flavonol 3-O-glucosyltransferase | 6.15 | 5.29 | 4.89 | 3.97 | G |
| Mtr.27374.1.S1_at | UDP-Glucosyltransferase | 6.82 | 6.33 | 4.94 | 5.39 | G |
| Mtr.37046.1.s1_at | UDP-Glucosyltransferase | 8.45 | 7.81 | 7.37 | 6.82 | G |
| Mtr.9669.1.s1_at | Transferase | 7.77 | 7.10 | 6.61 | 6.35 | G |
| Mtr.27554.1.s1_at | Transferase | 6.94 | 6.37 | 5.83 | 5.23 | G |
| Mtr.29306.1.s1_at | Transferase | 8.30 | 8.92 | 7.19 | 7.58 | G |
| Mtr.10626.1.s1_at | Flavonol synthase/flavanone 3-hydroxylase | 11.62 | 11.40 | 10.88 | 10.21 | G |
| Mtr.14782.1.s1_at | Flavonoid biosynthetic process DMR6 | 5.54 | 5.20 | 4.17 | 4.75 | G |
| Anthocyanin pathway | ||||||
| Mtr.31382.1.s1_at | Dihydroflavonol 4-reductase | 7.99 | 8.72 | 8.97 | 7.72 | G×N |
| Mtr.30762.1.s1_at | Anthocyanin 5-aromatic acyltransferase | 4.90 | 5.37 | 6.31 | 6.03 | G |
| Mtr.43878.1.s1_at | Anthocyaninless2 transcription factor | 7.83 | 6.97 | 6.29 | 5.95 | G |
| Mtr.35206.1.s1_at | Anthocyaninless2 transcription factor | 6.41 | 5.59 | 4.65 | 4.34 | G |
| Mtr.2481.1.S1_at | Protein Transparent Testa 12 | 2.87 | 3.62 | 3.34 | 5.03 | G N |