Fig. 6.

Expression levels of the endogenous carotenoid biosynthetic genes in endosperms from the transgenic and control wheat lines. Gene expression levels were measured by qRT-PCR and are determined relative to the transcript levels of the constitutively expressed β-actin gene in the same samples. Expression levels of these genes for the transformed lines are given as expression levels relative to the values for the non-transformed control line Bobwhite. qPCR results for each gene were performed for three biological replicates with three technical repeats each, and all data are shown as means±standard error of the mean.