Table 1.

Generation of transgenic wheat plants overexpressing bacterial CrtI and/or CrtB

Transgenic linea	Constructs	Gene(s) of interest	Selectable marker gene	No. regenerated plants	No. PCR-positive plants
OEI	pU-I, pAHC20	CrtI	bar	11	7
OEB	pTP-B, pAHC20	CrtB	bar	14	5
OEIB	pTP-B, pU-I, pAHC20	CrtB, CrtI	bar	11	3
VC	pAHC20	–	bar	28	11

^a VC is a wheat line transformed with plasmid pAHC20, which is used as transgenic control line; OEI is a line expressing CrtI; OEB is a line expressing CrtB, and OEIB has co-expression of CrtI and CrtB.