Table 2.
Transgene expression level, carotenoid content, 100-grain weight, and number of seeds per spike in T3 transgenic lines
| Transgenic line (T3 generation) | Relative transcript levelsa | Total carotenoids (μg g–1 of dry seed weight)b | Provitamin A (μg g–1 of dry seed weight)b | 100-Grain weight (g) | No. seeds per spike | |
|---|---|---|---|---|---|---|
| crtB | crtI | |||||
| BWc | 0 | 0 | 0.58±0.02 | 0.05±0.01 | 4.01±0.02 | 36.00±1.53 |
| OIE-1 | 0 | 0.027±0.002 | 0.65±0.02 | 0.18±0.01 | 3.84±0.12 | 33.67±1.76 |
| OEB-4 | 0.078±0.003 | 0 | 2.32±0.08 | 1.37±0.05 | 3.89±0.09 | 34.33±2.33 |
| OEIB-2 | 0.091±0.004 | 0.037±0.002 | 4.06±0.49 | 3.21±0.37 | 3.96±0.06 | 35.33±0.88 |
The data represent mean values±standard error of the mean and are derived from at least three independent plants per line.
a Relative transcript levels (normalized with respect to the β-actin transcript) were determined by qPCR.
b Total carotenoids (sum of all carotenoid content measured) and provitamin A content (sum of α-carotene, β-cryptoxanthin, β-carotene,) were determined by HPLC analysis.
c Bobwhite (BW) is shown as a control.